Particle Bombardment of Apple Leaf Explants Influences Adventitious Shoot Formation
نویسندگان
چکیده
منابع مشابه
Improving adventitious shoot regeneration from cultured leaf explants of Petunia hybrida using thidiazuron
The effect of various concentrations of thidiazuron (TDZ) with or without 2.7 μM of α-naphthalene acetic acid (NAA) on adventitious shoot formation of two Petunia hybrida cultivars was studied. Seeds from ‘Daddy Blue’ and ‘Dreams White’ cultivars were germinated in vitro. Expanded leaves from both seedlings and regenerated shoots were taken as explants. Explants were placed on MS media supplied...
متن کاملStudy of Direct Adventitious Shoot Regeneration in Pomegranate ( Punicagranatum cv. MalasSaveh ) through Cotyledonary Explants
Punicagranatum cv. Malassaveh is one of the most desirable commercial cultivars in Iran. In thisstudy some experiments were conducted to introduce the best hormonal treatments for directadventitious shoot regeneration from mentioned cultivar via cotyledonary explants. For thispurpose, both liquid and agarMurashige and Skoog (1962) (MS) medium supplemented with BAat 2, 4.5, 9, 13 μM alone and in...
متن کاملCytosine methylation occurs in a CDC48 homologue and a MADS-box gene during adventitious shoot induction in Petunia leaf explants.
The DNA methylase inhibitors, 5-azacytidine and 5-aza-2'-deoxycytidine inhibited adventitious shoot induction in Petunia leaf cultures. Cytosine methylation status at CCGG sites in shoot- and callus-inducing culture treatments was analysed by coupled restriction enzyme digestion (HpaII or MspI) and random amplification. Two differentially methylated genomic DNA bands from the PCR products were ...
متن کاملIndirect shoot organogenesis from leaf explants of Adhatoda vasica Nees
A novel protocol for indirect shoot organogenesis of Adhatoda vasica was developed using petiole explants derived from mature shrubby plants. Media with concentrations of cytokinins in combination with auxins were used to induce callus formation in two explants types: petiole and leaf segment. The frequency of callus formation from petiole and leaf segment explants on Murashige and Skoog (MS) b...
متن کاملIn vitro shoot organogenesis from Eucalyptus sp. leaf explants
Background In vitro organogenesis is one of the key techniques associated with genetic transformation, as it determines the successful regeneration of transgenic plants after co-cultivation with bacteria. Therefore, the development of efficient regeneration protocols is the most critical step in developing genetic transformation. Protocols have been developed for several species of eucalyptus. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: HortScience
سال: 1994
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.29.12.1536